Conformational Changes in the Chromatin Structure of Human Peripheral Blood Mononuclear Cells Exposed to Low Dose Radiation
نویسنده
چکیده
Ionizing radiations are known to challenge the integrity of the genome, by inducing several lesions like double strand breaks, single strand breaks and oxidative base damages in the DNA. Eukaryotic DNA is packaged inside the nucleus in the form of chromatin, which is formed by the interaction of histone proteins with DNA. Higher order chromatin structure acts as a barrier to the detection and repair of DNA damage. Modulations in chromatin structure are central in cellular responses to radiation-induced DNA damage. Such conformation changes may be monitored, by using a tool that studies the dynamic changes in size and shape of particles due to conformational modifications. Dynamic Light Scattering (DLS) is a sensitive and non-invasive tool, to study the dynamics of biomolecules in solution. In the present study, Dynamic Light Scattering (DLS) was used to analyze radiation-induced conformational changes in the structure of native chromatin.
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